Mmp 9 Antibody Cell Signaling

Add protein a agarose 10 30 µl of 50 bead slurry.

Mmp 9 antibody cell signaling.

In this product review i report results that the anti mouse mmp 9 antibody from cell signaling showed unsurpassed performance in detecting the protein in both western blotting and immunofluroscence microscopy. Incubate with rotation overnight at 4 c. Microcentrifuge for 30 sec at 4 c. Add primary antibody at the appropriate dilution as recommended in the product datasheet to 200 µl cell lysate at 1 mg ml.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lys554 of human mmp 2 protein. Antibodies are purified by protein a and peptide affinity chromatography. Background the matrix metalloproteinases mmps are a family of proteases that target many extracellular proteins including other proteases growth factors cell surface receptors and adhesion. Once synthesized mmp exists as a latent proenzyme.

Maximum mmp activity requires proteolytic cleavage to generate active mmps by releasing the inhibitory propeptide domain from the full length protein 5. Background the matrix metalloproteinases mmps are a family of proteases that target many extracellular proteins including other proteases growth factors cell surface receptors and adhesion. Anti rabbit igg hrp linked antibody 7074 was used as secondary antibody. Mmp 9 is a matrix metalloprotease produced from immune cells including macrophages and neutrophils which plays important roles in wound healing immunity and angiogenesis.

Cell signaling technology the mmp 9 13667 rabbit antibody is specific and with high affinity toward its target. It was detected by western blot in total lysate of human primary leukemia blasts. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding phe542 of human mmp 9 protein. Western blot analysis was performed using mmp 2 d4m2n rabbit mab as primary antibody.

One mechanism of mmp regulation is transcriptional 5. Immunoprecipitation of mmp 2 protein from u 118mg cell extracts. 2001 curr opin cell biol 13 534 40. Matrix metalloproteinase 9 92 kda gelatinase 92 kda type iv collagenase gelatinase b mmp9 human.

Mmp 13 is a member of a subgroup of collagenases including mmp 1 mmp 8 and mmp 18 that play an even more important function targeting fibrillar collagen. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lys115 of human mmp 9. Mmp9 in a mouse brain tumor model 1 write a review. Lane 1 is 10 input lane 2 is rabbit da1e mab igg xp isotype control 3900 and lane 3 is mmp 2 d4m2n rabbit mab.

Incubate with rotation for 1 3 hr at 4 c.